Transfer Buffer

 

WSE-7210EzFastBlot HMW 

Purpose and Application
  • Speedy transfer for high molecular weight proteins (200kDa <)

  • MeOH, Acid, Detergent free

  • Effective transfer of high molecules (MW200kDa)

  • The time to complete blotting is 15-30min, Greater time shortening 

  • 5x concentrated reagent-Dilute with distilled water before use

Reagents Luminol Substrate for HRP Detection


WSE-7120EzWestLumi plus

High Sensitive Peroxidase Substrate (Luminol) 

Purpose and Application
  • EzWestLumi is a chemiluminescent substrate to detect horseradish peroxidase (HRP) on western blotting membrane

  • A luminol substrate for chemiluminescent detection of horseradish peroxidase (HRP) 

  • High signal intensity and sensitivity

  • Wide dynamic range is suitable for detection of various concentrations of protein

  • Fast luminescent reaction allows to detect signals immediately

  • Economy; good cost performance 

Purpose and Application
  • Use for luminescence detection by HRP in western blotting.

  • 1 bottle type

  • Luminescence substrate for HRP(Horse Radish Peroxydase)

  • Keeping a luminescence for hour

  • Lower cost=38Yen/mL

  • Wide dynamic rage

  • Better Linearity 

Stripping Reagent


WSE-7240 EzReprobe

Purpose and Application
  • EzReprobe is a stripping reagent to remove the 1st and 2nd antibody from the membrane after western blotting reaction

  • Repeatable stripping and reprobing reaction

  • Less damage to the antigen on the blot

  • Repeatable stripping and reprobing reaction

  • Ready to use

  • Soak the used blot in EzReprobe and incubate for 10 min at room temperature

  • Economical; good cost performance

  •  Toxic substances FREE ( like β-mercaptoethanol)

  • Efficient stripping performance
    Rabbit monochlonal antibody which is generally shown high titer can be removed.

1. Immense membrane after emission detection in EzReprobe solution and shake it  to incubate for 15 min at room temperature.

    *Incubation time depends on antibody titer.

    *In the case of antibody having high antibody titer or difficult to come off, incubation time should be extended.

2. After cleaning with wash buffer, the step after blocking is performed for antigen-antibody reaction.

 
       Example data

        Even rabbit monoclonal antibody having high antibody titer can be stripped with EzReprobe. 

        Also, denaturation of antigen is minimized.

  • Sub band of human GAPDH is not detected at 4th step with EzReprobe.

  • EzReprobe has a large amount of expression of antigen such as GAPDH, and stripping can be performed beautifully under the condition which antibody titer is high if reaction time of stripping is extended.

 

*Red arrow head: Shows the band of target antigen of each step.

*Grey arrow head: Shows the band result from antibody cannot be peeled off by stripping operation.

*Non-Treated: Shake it to incubate at room temperature with wash buffer (TBST)

*Handmade:  Shake it to incubate for 30 min at 50 ℃ (refer to Kaufmann et al., 1987)

*The other 3 reagents and EzReprobe: Shake it to incubate for 10 min at room temperature

CONTACT INFO

 

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